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Dual regulation of the T-type Ca(2+) current by serum albumin and beta-estradiol in mammalian spermatogenic cells.
Espinosa, F; López-González, I; Muñoz-Garay, C; Felix, R; De la Vega-Beltrán, J L; Kopf, G S; Visconti, P E; Darszon, A.
Afiliación
  • Espinosa F; Department of Genetics and Molecular Physiology, Institute of Biotechnology, UNAM, Avenida Universidad 2001, Cuernavaca, Mexico.
FEBS Lett ; 475(3): 251-6, 2000 Jun 23.
Article en En | MEDLINE | ID: mdl-10869566
This study provides evidence for a novel mechanism of voltage-gated Ca(2+) channel regulation in mammalian spermatogenic cells by two agents that affect sperm capacitation and the acrosome reaction (AR). Patch-clamp experiments demonstrated that serum albumin induced an increase in Ca(2+) T current density in a concentration-dependent manner, and significant shifts in the voltage dependence of both steady-state activation and inactivation of the channels. These actions were not related to the ability of albumin to remove cholesterol from the membrane. In contrast, beta-estradiol significantly inhibited Ca(2+) channel activity in a concentration-dependent and essentially voltage-independent fashion. In mature sperm this dual regulation may influence capacitation and/or the AR.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espermatozoides / Albúmina Sérica / Calcio / Canales de Calcio Tipo T / Estradiol Límite: Animals Idioma: En Revista: FEBS Lett Año: 2000 Tipo del documento: Article País de afiliación: México Pais de publicación: Reino Unido
Buscar en Google
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PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espermatozoides / Albúmina Sérica / Calcio / Canales de Calcio Tipo T / Estradiol Límite: Animals Idioma: En Revista: FEBS Lett Año: 2000 Tipo del documento: Article País de afiliación: México Pais de publicación: Reino Unido