Coexpression of rat P2X2 and P2X6 subunits in Xenopus oocytes.
J Neurosci
; 20(13): 4871-7, 2000 Jul 01.
Article
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| MEDLINE
| ID: mdl-10864944
Transcripts for P2X(2) and P2X(6) subunits are present in rat CNS and frequently colocalize in the same brainstem nuclei. When rat P2X(2) (rP2X(2)) and rat P2X(6) (rP2X(6)) receptors were expressed individually in Xenopus oocytes and studied under voltage-clamp conditions, only homomeric rP2X(2) receptors were fully functional and gave rise to large inward currents (2-3 microA) to extracellular ATP. Coexpression of rP2X(2) and rP2X(6) subunits in Xenopus oocytes resulted in a heteromeric rP2X(2/6) receptor, which showed a significantly different phenotype from the wild-type rP2X(2) receptor. Differences included reduction in agonist potencies and, in some cases (e.g., Ap(4)A), significant loss of agonist activity. ATP-evoked inward currents were biphasic at the heteromeric rP2X(2/6) receptor, particularly when Zn(2+) ions were present or extracellular pH was lowered. The pH range was narrower for H(+) enhancement of ATP responses at the heteromeric rP2X(2/6) receptor. Also, H(+) ions inhibited ATP responses at low pH levels (
Asunto(s)
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Adenosina Trifosfato
/
Receptores Purinérgicos P2
Límite:
Animals
Idioma:
En
Revista:
J Neurosci
Año:
2000
Tipo del documento:
Article
País de afiliación:
Reino Unido
Pais de publicación:
Estados Unidos
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Adenosina Trifosfato
/
Receptores Purinérgicos P2
Límite:
Animals
Idioma:
En
Revista:
J Neurosci
Año:
2000
Tipo del documento:
Article
País de afiliación:
Reino Unido
Pais de publicación:
Estados Unidos