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Specific protein-protein interaction between basic helix-loop-helix transcription factors and homeoproteins of the Pitx family.
Poulin, G; Lebel, M; Chamberland, M; Paradis, F W; Drouin, J.
Afiliación
  • Poulin G; Laboratoire de Génétique Moléculaire, Institut de Recherches Cliniques de Montréal, Montréal, Québec H2W 1R7, Canada.
Mol Cell Biol ; 20(13): 4826-37, 2000 Jul.
Article en En | MEDLINE | ID: mdl-10848608
Homeoproteins and basic helix-loop-helix (bHLH) transcription factors are known for their critical role in development and cellular differentiation. The pituitary pro-opiomelanocortin (POMC) gene is a target for factors of both families. Indeed, pituitary-specific transcription of POMC depends on the action of the homeodomain-containing transcription factor Pitx1 and of bHLH heterodimers containing NeuroD1. We now show lineage-restricted expression of NeuroD1 in pituitary corticotroph cells and a direct physical interaction between bHLH heterodimers and Pitx1 that results in transcriptional synergism. The interaction between the bHLH and homeodomains is restricted to ubiquitous (class A) bHLH and to the Pitx subfamily. Since bHLH heterodimers interact with Pitx factors through their ubiquitous moiety, this mechanism may be implicated in other developmental processes involving bHLH factors, such as neurogenesis and myogenesis.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Transactivadores / Proteínas de Homeodominio / Proteínas de Unión al ADN Límite: Animals Idioma: En Revista: Mol Cell Biol Año: 2000 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Transactivadores / Proteínas de Homeodominio / Proteínas de Unión al ADN Límite: Animals Idioma: En Revista: Mol Cell Biol Año: 2000 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos