Synthesis and properties of uniquely modified oligoribonucleotides: yeast tRNA(Phe) fragments with 6-methyluridine and 5,6-dimethyluridine at site-specific positions.
Nucleosides Nucleotides Nucleic Acids
; 19(3): 515-31, 2000 Mar.
Article
en En
| MEDLINE
| ID: mdl-10843489
The phosphoramidites of 6-methyluridine and 5,6-dimethyluridine were synthesized and the modified uridines site-selectively incorporated into heptadecamers corresponding in sequence to the yeast tRNA(Phe) anticodon and TpsiC domains. The oligoribonucleotides were characterized by NMR, MALDI-TOF MS and UV-monitored thermal denaturations. The 6-methylated uridines retained the syn conformation at the polymer level and in each sequence location destabilized the RNAs compared to that of the unmodified RNA. The decrease in RNA duplex stability is predictable. However, loss of stability when the modified uridine is in a loop is sequence context dependent, and can not, at this time, be predicted from the location in the loop.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oligorribonucleótidos
/
Uridina
/
ARN de Transferencia de Fenilalanina
Idioma:
En
Revista:
Nucleosides Nucleotides Nucleic Acids
Asunto de la revista:
BIOQUIMICA
Año:
2000
Tipo del documento:
Article
País de afiliación:
Polonia
Pais de publicación:
Estados Unidos