Viability of plant cell suspensions exposed to homogeneous ultrasonic fields of different energy density and wave type.
Ultrasonics
; 38(1-8): 629-32, 2000 Mar.
Article
en En
| MEDLINE
| ID: mdl-10829740
Exposure of Petunia hybrida cell suspensions to ultrasound at a frequency of 2.43 MHz in a standing wave field at an energy density of 70 Jm-3 (pressure amplitude of 0.78 MPa) decreased their mean viability to 35% after 20 min of sonication. A comparison of propagating wave and standing wave treatments at equal frequency (2.15 MHz) and energy density (8.5 Jm-3) showed, in the first case, a rapid decline in mean viability of cells (to 30% after 10 min of sonication) and, in the second case, a retaining of the initial viability (95%), respectively. Cells sonicated 4 days after subculture were more sensitive than cells sonicated 2 or 6 days after transfer to new culture medium. It was concluded that cellular viability depends primarily on the acoustic energy density, the exposure time, and the mechanical properties of the cells determined by age. As a consequence of the trapping of cells in the anti-node planes of the standing wave, propagating wave fields reduced cellular viability compared with standing wave fields at equal energy density.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ultrasonido
/
Células Vegetales
Idioma:
En
Revista:
Ultrasonics
Año:
2000
Tipo del documento:
Article
Pais de publicación:
Países Bajos