Detection of progesterone receptor transcript in human spermatozoa.

Sachdeva, G; Shah, C A; Kholkute, S D; Puri, C P

Sachdeva, G; Shah, C A; Kholkute, S D; Puri, C P.

Afiliación

Sachdeva G; Institute for Research in Reproduction, Indian Council of Medical Research, Parel, Mumbai 400 012, India.

Biol Reprod ; 62(6): 1610-4, 2000 Jun.

Article en En | MEDLINE | ID: mdl-10819762

RESUMEN

The present study, to our knowledge, is the first to demonstrate presence of progesterone receptor (PR) transcript in human spermatozoa. The study shows the presence of low copy number PR mRNA in mature human spermatozoa. The PR transcript in spermatozoa was detected by reverse transcriptase-polymerase chain reaction using primers specific for the hormone binding domain and the DNA binding domain of the conventional uterine PR. Further, the cDNA sequence of the partial PR transcript from spermatozoa was found to be identical to the region spanning nucleotides 2694 to 3230 of the conventional PR full-length cDNA sequence. This study also indirectly suggests that the PR protein indeed is an intrinsic sperm protein and is not acquired through proteinaceous secretions of accessory reproductive organs.

Asunto(s)

ARN Mensajero/análisis; Receptores de Progesterona/genética; Espermatozoides/química; Secuencia de Bases; Sitios de Unión; ADN/química; ADN/metabolismo; Humanos; Masculino; Datos de Secuencia Molecular; Progesterona/metabolismo; Reacción en Cadena de la Polimerasa de Transcriptasa Inversa

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espermatozoides / ARN Mensajero / Receptores de Progesterona Tipo de estudio: Diagnostic_studies Límite: Humans / Male Idioma: En Revista: Biol Reprod Año: 2000 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos

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