Cervical lesions are associated with human papillomavirus type 16 intratypic variants that have high transcriptional activity and increased usage of common mammalian codons.

Bible, J M; Mant, C; Best, J M; Kell, B; Starkey, W G; Shanti Raju, K; Seed, P; Biswas, C; Muir, P; Banatvala, J E; Cason, J

Bible, J M; Mant, C; Best, J M; Kell, B; Starkey, W G; Shanti Raju, K; Seed, P; Biswas, C; Muir, P; Banatvala, J E; Cason, J.

Afiliación

Bible JM; The Richard Dimbleby Laboratory of Cancer Virology, Guy's, King's College and St Thomas' Medical and Dental Schools, King's College London, St Thomas' Campus, London, UK.

J Gen Virol ; 81(Pt 6): 1517-27, 2000 Jun.

Article en En | MEDLINE | ID: mdl-10811935

RESUMEN

Human papillomavirus type 16 (HPV-16) is a major cause of cervical neoplasia, but only a minority of HPV-16 infections result in cancer. Whether particular HPV-16 variants are associated with cervical disease has not yet been clearly established. An investigation of whether cervical neoplasia is associated with infection with HPV-16 intratypic variants was undertaken by using RFLP analyses in a study of 100 HPV-16 DNA-positive women with or without neoplasia. RFLP variant 2 was positively associated [odds ratio (OR)=2.57] and variant 5 was negatively associated with disease (OR=0.2). Variant 1, which resembles the reference isolate of HPV-16, was found at a similar prevalence among those with and without neoplasia. Variants 1 and 2 were also more likely to be associated with detectable viral mRNA than variant 5 (respectively P=0.03 and P=0.00). When HPV-16 E5 ORFs in 50 clones from 36 clinical samples were sequenced, 19 variant HPV-16 E5 DNA sequences were identified. Twelve of these DNA sequences encoded variant E5 amino acid sequences, 10 of which were novel. Whilst the associations between HPV-16 E5 RFLP variants and neoplasia could not be attributed to differences in amino acid sequences, correlation was observed in codon usage. DNA sequences of RFLP variant 2 (associated with greatest OR for neoplasia) had a significantly greater usage of common mammalian codons compared with RFLP pattern 1 variants.

Asunto(s)

Cuello del Útero/virología; Variación Genética; Proteínas Oncogénicas Virales/genética; Papillomaviridae/genética; Infecciones por Papillomavirus/virología; Transcripción Genética; Infecciones Tumorales por Virus/virología; Displasia del Cuello del Útero/virología; Neoplasias del Cuello Uterino/virología; Secuencia de Aminoácidos; Secuencia de Bases; Cuello del Útero/patología; Codón; ADN Viral; Femenino; Humanos; Datos de Secuencia Molecular; Infecciones por Papillomavirus/patología; Infecciones Tumorales por Virus/patología; Neoplasias del Cuello Uterino/patología; Displasia del Cuello del Útero/patología

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Papillomaviridae / Transcripción Genética / Infecciones Tumorales por Virus / Variación Genética / Displasia del Cuello del Útero / Neoplasias del Cuello Uterino / Cuello del Útero / Proteínas Oncogénicas Virales / Infecciones por Papillomavirus Tipo de estudio: Risk_factors_studies Límite: Female / Humans Idioma: En Revista: J Gen Virol Año: 2000 Tipo del documento: Article Pais de publicación: Reino Unido

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