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Expression of cyclooxygenase-2 in transitional cell carcinoma of the urinary bladder in dogs.
Khan, K N; Knapp, D W; Denicola, D B; Harris, R K.
Afiliación
  • Khan KN; Searle Research and Development, Skokie, IL 60077, USA.
Am J Vet Res ; 61(5): 478-81, 2000 May.
Article en En | MEDLINE | ID: mdl-10803639
OBJECTIVE: To evaluate expression of cyclooxygenase (COX)-1 and COX-2 in the urinary bladder epithelium of clinically normal dogs and in transitional cell carcinoma cells of dogs. ANIMALS: 21 dogs with transitional cell carcinoma of the urinary bladder and 8 dogs with clinically normal urinary bladders. PROCEDURE: COX-1 and COX-2 were evaluated by use of isoform-specific antibodies with standard immunohistochemical methods. RESULT: COX-1, but not COX-2, was constitutively expressed in normal urinary bladder epithelium; however, COX-2 was expressed in neoplastic epithelium in primary tumors and in metastatic lesions of all 21 dogs and in new proliferating blood vessels in 3 dogs. Also, COX-1 was expressed in the neoplastic cells. CONCLUSIONS AND CLINICAL RELEVANCE: Lack of expression of COX-2 in normal bladder epithelium and its substantial expression in transitional cell carcinoma cells suggest that this isoform may be involved in tumor cell growth. Inhibition of COX-2 is a likely mechanism of the antineoplastic effects of non steroidal antiinflammatory drugs.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Vejiga Urinaria / Carcinoma de Células Transicionales / Regulación Neoplásica de la Expresión Génica / Prostaglandina-Endoperóxido Sintasas / Enfermedades de los Perros / Isoenzimas Límite: Animals Idioma: En Revista: Am J Vet Res Año: 2000 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Vejiga Urinaria / Carcinoma de Células Transicionales / Regulación Neoplásica de la Expresión Génica / Prostaglandina-Endoperóxido Sintasas / Enfermedades de los Perros / Isoenzimas Límite: Animals Idioma: En Revista: Am J Vet Res Año: 2000 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos