P2X3 receptor in injured human sensory neurons.
Neuroreport
; 11(5): 993-6, 2000 Apr 07.
Article
en En
| MEDLINE
| ID: mdl-10790870
The ATP-gated cation channel P2X3 is expressed selectively by rat sensory neurones, and may play a role in nociception by binding ATP released from damaged or inflamed tissues. However, the distribution of this channel in human sensory neurons is not known. Using a specific antibody, we have demonstrated intense P2X3 immunoreactivity within a subset (60%) of small/medium diameter sensory neurones and fine nerve fibres in intact post-mortem human dorsal root ganglia (DRG). Co-localization studies showed < 15% overlap with the trkA immunostaining in DRG, indicating that P2X3 was expressed predominantly in sensory neurons that are also isolectin B4 positive. There was a significant decrease in numbers of P2X3-like immunoreactive neurons in human DRG after central axotomy (to 36%), similar to the decrease in rat DRG after peripheral axotomy. However, Western blotting demonstrated a specific 66 kDa band in human DRG and peripheral organs, including intestine, where histochemistry showed P2X3 immunoreactivity in myenteric plexus neurons. Thus P2X3 antagonists may be analgesic, but are unlikely to have a selective effect on pain in humans.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Receptores Purinérgicos P2
/
Ganglios Espinales
/
Degeneración Nerviosa
/
Neuronas Aferentes
Límite:
Adult
/
Humans
Idioma:
En
Revista:
Neuroreport
Asunto de la revista:
NEUROLOGIA
Año:
2000
Tipo del documento:
Article
Pais de publicación:
Reino Unido