Extension of cell life-span and telomere length in animals cloned from senescent somatic cells.
Science
; 288(5466): 665-9, 2000 Apr 28.
Article
en En
| MEDLINE
| ID: mdl-10784448
The potential of cloning depends in part on whether the procedure can reverse cellular aging and restore somatic cells to a phenotypically youthful state. Here, we report the birth of six healthy cloned calves derived from populations of senescent donor somatic cells. Nuclear transfer extended the replicative life-span of senescent cells (zero to four population doublings remaining) to greater than 90 population doublings. Early population doubling level complementary DNA-1 (EPC-1, an age-dependent gene) expression in cells from the cloned animals was 3.5- to 5-fold higher than that in cells from age-matched (5 to 10 months old) controls. Southern blot and flow cytometric analyses indicated that the telomeres were also extended beyond those of newborn (<2 weeks old) and age-matched control animals. The ability to regenerate animals and cells may have important implications for medicine and the study of mammalian aging.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bovinos
/
Telómero
/
Senescencia Celular
/
Clonación de Organismos
/
Proteínas del Ojo
/
Técnicas de Transferencia Nuclear
/
Factores de Crecimiento Nervioso
Límite:
Animals
Idioma:
En
Revista:
Science
Año:
2000
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos