Quantitative high-performance liquid chromatographic determination of acrolein in plasma after derivatization with Luminarin 3.
J Chromatogr B Biomed Sci Appl
; 739(2): 239-46, 2000 Mar 10.
Article
en En
| MEDLINE
| ID: mdl-10755368
A rapid, sensitive and specific high-performance liquid chromatographic method for the quantification of acrolein (1), one of the toxic metabolites of oxazaphosphorine alkylating agents (cyclophosphamide and ifosfamide) was developed. Condensation of acrolein with Luminarin 3 afforded a fluorescent derivative that could be specifically detected and quantified. Chromatographic conditions involved a C18 RP column Uptisphere and a gradient elution system to optimize resolution and time analysis. The method showed high sensitivity with a limit of detection of 100 pmol/ml and a limit of quantification of 300 pmol/ml. This technique is particularly suitable for pharmacokinetic studies on plasma of oxazaphosphorine-receiving patients.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Quinolizinas
/
Acroleína
/
Cromatografía Líquida de Alta Presión
/
Colorantes Fluorescentes
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Chromatogr B Biomed Sci Appl
Asunto de la revista:
QUIMICA CLINICA
Año:
2000
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Países Bajos