Purification, crystallization and preliminary X-ray analysis of haemoglobin I from the armoured catfish Liposarcus anisitsi.

Smarra, A L; de Azevedo, W F; Fadel, V; Delatorre, P; Dellamano, M; Colombo, M F; Bonilla-Rodriguez, G O

Smarra, A L; de Azevedo, W F; Fadel, V; Delatorre, P; Dellamano, M; Colombo, M F; Bonilla-Rodriguez, G O.

Afiliación

Smarra AL; Departamento de Física, Instituto de Biociências, Letras e Ciências Exatas, UNESP, São José do Rio Preto, SP 15054-000, Brazil.

Acta Crystallogr D Biol Crystallogr ; 56(Pt 4): 495-7, 2000 Apr.

Article en En | MEDLINE | ID: mdl-10739931

RESUMEN

Liposarcus anisitsi is an armoured catfish that presents accessorial air oxygenation through a modified stomach, which allows this species to survive in waters with very low oxygen content. Analysis of its haemolysate has shown the presence of four haemoglobins; this work focuses on the main component, haemoglobin I. It has been crystallized in two different forms and X-ray diffraction data have been collected to 2.77 and 2.86 A resolution using synchrotron radiation. Crystals were determined to belong to the space groups C2 and P2(1) and preliminary structural analysis revealed the presence of one tetramer in the asymmetric unit in both crystal forms. The structure was determined using a standard molecular-replacement technique.

Asunto(s)

Hemoglobinas Anormales/química; Hemoglobinas; Animales; Bagres; Cristalización; Cristalografía por Rayos X; Hemoglobinas Anormales/aislamiento & purificación; Sincrotrones

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hemoglobinas / Hemoglobinas Anormales Límite: Animals Idioma: En Revista: Acta Crystallogr D Biol Crystallogr Año: 2000 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos

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