Role of spleen macrophages in the clearance of scrapie agent early in pathogenesis.
J Pathol
; 190(4): 495-502, 2000 Mar.
Article
en En
| MEDLINE
| ID: mdl-10700001
The involvement of spleen macrophages in the early stages of scrapie pathogenesis was studied by applying the 'macrophage-suicide technique' to scrapie-infected mice. This method comprises critically the intravenous administration to mice of dichloromethylene disphosphonate encapsulated into liposomes. Depletion of spleen macrophages before scrapie infection induced an increased amount of scrapie inoculum in the spleen, consequently leading to accelerated scrapie agent replication in the early phase of pathogenesis, as followed by PrPres accumulation, a specific hallmark of scrapie. The same effect was observed when spleen macrophages were depleted just before the beginning of scrapie agent replication. These findings suggest that macrophages may partly control scrapie infection in peripheral tissues by sequestration of the scrapie inoculum and may thus impair early scrapie agent replication in the spleen. In addition to macrophages, most follicular dendritic cells and B lymphocytes, which are thought to support scrapie agent replication, were also transiently depleted by dichloromethylene disphosphonate administration. This suggests that a compensatory mechanism is sufficient to ensure the persistence of infection in these early stages of pathogenesis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Scrapie
/
Bazo
/
Macrófagos
Tipo de estudio:
Etiology_studies
Límite:
Animals
Idioma:
En
Revista:
J Pathol
Año:
2000
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Reino Unido