Antisense human neuroglia related cell adhesion molecule hNr-CAM, reduces the tumorigenic properties of human glioblastoma cells.
Anticancer Res
; 19(6B): 4947-53, 1999.
Article
en En
| MEDLINE
| ID: mdl-10697494
BACKGROUND: Human Nr-CAM (Neuroglia related Cell Adhesion Molecule) is over expressed in glioblastoma multiforme tissue (GMT) as compared to normal brain tissue (NBT). MATERIALS AND METHODS: We transfected a human glioblastoma cell line (2020-CRL) with a vector that overexpresses antisense hNr-CAM using a CMVpromoter. RESULTS: Antisense hNr- CAM caused reduction in the native hNr-CAM expression, changed cell morphology, reduced the cell proliferation rate and lengthening of the cell cycle. Furthermore, antisense hNr-CAM overexpression in these cells caused extensive reduction in the number of soft agar colonies and invasion through extra cellular matrix (ECM) gel in vitro. Subcutaneous injection of antisense hNr-CAM overexpressing glioblastoma cells into nude mice caused complete inhibition of tumor formation as compared to vector only transfected cells. Intra-tumoral inoculation of antisense hNr-CAM expressing plasmid also caused slow tumor growth in nude mice in vivo. CONCLUSION: On the basis of these results, we conclude that hNr-CAM is a valid target for potential gene therapy of glioblastoma tumors.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Encefálicas
/
Moléculas de Adhesión Celular Neuronal
/
Moléculas de Adhesión Celular
/
Oligonucleótidos Antisentido
/
Glioblastoma
Límite:
Animals
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Female
/
Humans
/
Middle aged
Idioma:
En
Revista:
Anticancer Res
Año:
1999
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Grecia