Spatial separation of parental genomes in preimplantation mouse embryos.
J Cell Biol
; 148(4): 629-34, 2000 Feb 21.
Article
en En
| MEDLINE
| ID: mdl-10684246
We have used two different experimental approaches to demonstrate topological separation of parental genomes in preimplantation mouse embryos: mouse eggs fertilized with 5-bromodeoxyuridine (BrdU)-labeled sperm followed by detection of BrdU in early diploid embryos, and differential heterochromatin staining in mouse interspecific hybrid embryos. Separation of chromatin according to parental origin was preserved up to the four-cell embryo stage and then gradually disappeared. In F1 hybrid animals, genome separation was also observed in a proportion of somatic cells. Separate nuclear compartments during preimplantation development, when extreme chromatin remodelling occurs, and possibly in some differentiated cell types, may be associated with epigenetic reprogramming.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Blastocisto
/
Núcleo Celular
/
Genoma
/
Padre
/
Madres
Límite:
Animals
Idioma:
En
Revista:
J Cell Biol
Año:
2000
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Estados Unidos