Simultaneous determination of polycarboxylic acids by capillary electrophoresis with a copper electrode.
J Chromatogr A
; 867(1-2): 261-9, 2000 Jan 21.
Article
en En
| MEDLINE
| ID: mdl-10670726
The simultaneous determination of polycarboxylic acids including oxalic acid, citric acid, malonic acid, malic acid, tartaric acid, aspartic acid and glutamic acid was achieved by capillary electrophoresis with a copper disk electrode (d = 200 microm). In the system. 0.2 mmol/l cetylpridinium bromide (CPB) was used as an electroosmotic flow (EOF) modifier to reverse the direction of EOF. The effects of the solution pH and CPB concentration on separation were evaluated to achieve the optimum separation conditions. At the working potential of +0.14 V (vs. saturated calomel electrode), the calibration curves for all polycarboxylic acids studied were linear with 2 approximately 3-orders of magnitude and all the detection limits (S/N = 3) were below 15 fmol except malonic acid. Furthermore, the oxalic and citric acids in urine were successfully separated and determined with high sensitivity.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ácidos Carboxílicos
/
Electroforesis Capilar
/
Cobre
/
Electrodos
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
J Chromatogr A
Año:
2000
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Países Bajos