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Angiogenesis inhibitor TNP-470: cytotoxic effects on human neoplastic cell lines.
Sedláková, O; Sedlák, J; Hunáková, L; Jakubíková, J; Duraj, J; Sulíková, M; Chovancová, J; Chorváth, B.
Afiliación
  • Sedláková O; Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Neoplasma ; 46(5): 283-9, 1999.
Article en En | MEDLINE | ID: mdl-10665843
Angiostatic substance TNP-470 displayed moderate cytotoxicity towards human leukemia HL-60, HL-60/ADR, HL-60/VCR and myeloma ARH77 cell lines with IC50 in the range 5-10 microM of concentrations and slightly higher IC50 for myeloma cell line U266. IC50 for ovarian CH-1, A2780 and A2780/ADR cell lines was in the range 10-15 microM with the exception of platinum-resistant SKOV3 cell line (more than 40 microM ). The IC50 values for MDA-MB-231 and MCF-7 breast carcinoma cell lines were 15 and 25 microM, respectively. In human hemopoietic neoplastic cell lines examined, TNP-470 induced the appearance of subpopulation with sub-G0 DNA content, suggesting the apoptosis-inducing potential of TNP-470 in these cells. No TNP-470-induced drug uptake modulation in drug-resistant leukemia cell line HL-60/VCR was observed. TNP-470 induced accumulation of cells in G0/G1 phase of cell cycle. There was no TNP-470-induced inhibition of MMP collagenase activity or MMP (MMP2 and MMP9) production in the human fibrosarcoma cells HT 1080 in vitro.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sesquiterpenos / Inhibidores de la Angiogénesis / Antibióticos Antineoplásicos Límite: Humans Idioma: En Revista: Neoplasma Año: 1999 Tipo del documento: Article Pais de publicación: Eslovaquia
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sesquiterpenos / Inhibidores de la Angiogénesis / Antibióticos Antineoplásicos Límite: Humans Idioma: En Revista: Neoplasma Año: 1999 Tipo del documento: Article Pais de publicación: Eslovaquia