Surface plasmon resonance (SPR) analysis of coagulation in whole blood with application in prothrombin time assay.
Biosens Bioelectron
; 14(8-9): 671-82, 1999 Dec.
Article
en En
| MEDLINE
| ID: mdl-10641287
It is previously shown that surface plasmon resonance (SPR) can be used to study blood plasma coagulation. This work explores the use of this technique for the analysis of tissue factor induced coagulation, i.e. prothrombin time (PT) analysis, of whole blood and plasma. The reference method was nephelometry. The prothrombin time analysis by SPR was performed by mixing two volumes of blood/plasma, one volume of thromboplastin, and one volume of CaCl2 solution directly on a sensor surface. The measurements show good agreement between nephelometry and SPR plasma analysis and also between SPR plasma and whole blood analysis. The effect of anticoagulant treatment on the clotting times was significant both quantitatively and qualitatively. The impact on the SPR signal of different physiological events in the coagulation process is discussed, and tentative interpretations of the sensorgram features are given. The major advantage of the SPR method compared to nephelometry is the possibility to perform analysis on whole blood instead of plasma. In conclusion, SPR is a promising method for whole blood coagulation analysis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tiempo de Protrombina
/
Coagulación Sanguínea
/
Resonancia por Plasmón de Superficie
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Humans
Idioma:
En
Revista:
Biosens Bioelectron
Asunto de la revista:
BIOTECNOLOGIA
Año:
1999
Tipo del documento:
Article
País de afiliación:
Suecia
Pais de publicación:
Reino Unido