Pattern formation and regulation of gene expressions in chick recombinant limbs.
Mech Dev
; 90(2): 167-79, 2000 Feb.
Article
en En
| MEDLINE
| ID: mdl-10640702
Recombinant limbs were performed by ensembling dissociated-reaggregated wing bud mesoderm inside an ectodermal hull. The zone of polarizing activity was excluded from the mesoderm used to perform the recombinant limbs (non-polarized recombinants), and grafted when desired (polarized recombinants). Reorganization of patterning progressively occurred in the newly formed progress zone under the influence of the apical ectodermal ridge (AER), explaining the proximo-distal gradient of morphogenesis observed in developed recombinant limbs. The AER, without the influence of the polarizing region (ZPA), was sufficient to direct outgrowth and appropriate proximo-distal patterning, as observed in the expression of the Hoxa-11 and Hoxa-13 genes. The development of the recombinant limbs coursed with symmetric AER and downregulation of Bmp expression in the mesoderm supporting a negative effect of Bmp signaling upon the apical ridge. The recombinant ectoderm maintained previously established compartments of gene expressions and organized a correct dorso-ventral patterning in the recombinant progress zone. Finally, the ZPA effect was only detected on Bmp expression and pattern formation along the antero-posterior axis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factor de Crecimiento Transformador beta
/
Proteínas Proto-Oncogénicas
/
Regulación del Desarrollo de la Expresión Génica
/
Tipificación del Cuerpo
/
Proteínas Aviares
Límite:
Animals
Idioma:
En
Revista:
Mech Dev
Asunto de la revista:
EMBRIOLOGIA
Año:
2000
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Irlanda