Selective changes in the protein-turnover rates and nature of growth induced in trout liver by long-term starvation followed by re-feeding.
Mol Cell Biochem
; 201(1-2): 1-10, 1999 Nov.
Article
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| MEDLINE
| ID: mdl-10630616
We report upon the effects of a cycle of long-term starvation followed by re-feeding on the liver-protein turnover rates and nature of protein growth in the rainbow trout (Oncorhynchus mykiss). We determined the protein-turnover rate and its relationship with the nucleic-acid concentrations in the livers of juvenile trout starved for 70 days and then re-fed for 9 days. During starvation the total hepatic-protein and RNA contents decreased significantly and the absolute protein-synthesis rate (A(S)) also fell, whilst the fractional protein-synthesis rate (K(S)) remained unchanged and the fractional protein-degradation rate (K(D)) increased significantly. Total DNA content, an indicator of hyperplasia, and the protein:DNA ratio, an indicator of hypertrophy, both fell considerably. After re-feeding for 9 days the protein-accumulation rates (K(G), A(G)) rose sharply, as did K(S), A(S), K(D)), protein-synthesis efficiency (K(RNA)) and the protein-synthesis rate/DNA unit (K(DNA)). The total hepatic protein and RNA contents increased but still remained below the control values. The protein:DNA and RNA:DNA ratios increased significantly compared to starved fish. These changes demonstrate the high response capacity of the protein-turnover rates in trout liver upon re-feeding after long-term starvation. Upon re-feeding hypertrophic growth increased considerably whilst hyperplasia remained at starvation levels.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Inanición
/
Proteínas
/
Oncorhynchus mykiss
/
Hígado
Límite:
Animals
Idioma:
En
Revista:
Mol Cell Biochem
Año:
1999
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Países Bajos