Techniques for measuring and manipulating free Ca2+ in the cytosol and organelles of neutrophils.
J Immunol Methods
; 232(1-2): 77-88, 1999 Dec 17.
Article
en En
| MEDLINE
| ID: mdl-10618511
Ca(2+) signalling in neutrophils is important for triggering and coordinating the behaviour of neutrophils. Fluorescent probes for cytosolic free Ca(2+) concentration, e.g., fura2 and fluo3, have been widely used in neutrophils. These probes can be used to monitor Ca(2+) in the cytosol, the nucleus, near the plasma membrane and theoretically within Ca(2+) storage organelles. The longer wavelength indicators, e.g., fluo3 and calcium green, can be used confocally to monitor subcellular Ca(2+) changes in the cytosol of neutrophils and in the nucleus. Confocal techniques also permit "impossible views" imaging of Ca(2+) and newer scanning techniques promise very fast temporal resolution. Techniques using chlortetracycline (CTC) and DiOC(6)(3) are also described for monitoring the position of Ca(2+) storage sites in neutrophils and for manipulating their activity. Thus, in this review, a spectrum of new (and older) optical techniques are presented which are useful for measuring, monitoring and manipulating cytosolic free Ca(2+) concentration and Ca(2+) storage in neutrophils. With these techniques, it is hoped that more insight will be gained into both the mechanism of and the consequences of Ca(2+) signalling in neutrophils.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Orgánulos
/
Calcio
/
Técnicas de Sonda Molecular
/
Citosol
/
Neutrófilos
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Immunol Methods
Año:
1999
Tipo del documento:
Article
Pais de publicación:
Países Bajos