Cytogenetic and DNA-fingerprint characterization of choriocarcinoma cell lines and a trophoblast/choriocarcinoma cell hybrid.
Cancer Genet Cytogenet
; 116(1): 16-22, 2000 Jan 01.
Article
en En
| MEDLINE
| ID: mdl-10616526
We report the successful fusion of human choriocarcinoma cells with normal human trophoblast cells to a choriocarcinoma/trophoblast hybrid. The hybrid cells ACH1P were derived from fusion of primary male trophoblast cells with the HGPRT-defective choriocarcinoma cell line AC1-1. The karyotypes of the parental choriocarcinoma cell line JEG-3, its HGPRT-defective mutant clones AC1-1, AC1-5, and AC1-9, and the choriocarcinoma/trophoblast hybrid ACH1P are presented, together with a detailed characterization of the AC1-specific chromosomal marker add(X)(q26) using conventional cytogenetic banding techniques and multiplex-fluorescence in situ hybridization (M-FISH). To our knowledge, this is the first report of a stably proliferating human cell hybrid of trophoblastic origin, providing a unique cell culture model to study trophoblast-related invasion and its underlying genetic mechanisms.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Trofoblastos
/
Neoplasias Uterinas
/
Coriocarcinoma
/
Dermatoglifia del ADN
/
Células Híbridas
/
Cariotipificación
Límite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
Cancer Genet Cytogenet
Año:
2000
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Estados Unidos