Cyclodextrins as activator and inhibitor of latent banana pulp polyphenol oxidase.
J Agric Food Chem
; 47(2): 518-23, 1999 Feb.
Article
en En
| MEDLINE
| ID: mdl-10563926
The effect of cyclodextrins (CDs) on o-diphenol oxidation catalyzed by banana polyphenol oxidase (PPO) was studied. The oxidation of dopamine, the natural substrate of banana, in the presence of cyclodextrins was unaffected, because this hydrophilic phenol does not form inclusion complexes with CDs. However, when a hydrophobic phenol such as tert-butylcatechol (TBC) was used, a marked inhibition was observed with beta-, hydroxypropyl-beta-, and maltosyl-beta- CDs. This inhibition was due to the complexation of TBC in the CD core, demonstrating that banana pulp PPO worked only toward free substrate and not toward the complex TBC-CDs. In addition, the effect of some inhibitors in the presence of CDs and dopamine as substrate was studied. Increasing concentrations of CDs, in the presence of two inhibitors (4-iodophenol and cinnamic acid) were able to activate the inhibited enzyme to reach the noninhibited level by complexing the inhibitors in the hydrophobic core of the CDs. This dual effect of CDs as activator and inhibitor was tested in crude banana pulp extracts, with surprising activation effects never before described being observed.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Catecol Oxidasa
/
Zingiberales
/
Activadores de Enzimas
/
Ciclodextrinas
/
Inhibidores Enzimáticos
Idioma:
En
Revista:
J Agric Food Chem
Año:
1999
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Estados Unidos