A novel model to study the dorsolateral migration of melanoblasts.
Mech Dev
; 89(1-2): 3-14, 1999 Dec.
Article
en En
| MEDLINE
| ID: mdl-10559475
Melanocytes derived from pluripotent neural crest cells migrate initially in the dorsolateral pathway between the ectoderm and dermomyotome. To understand the role of specific proteins involved in this cell migration, we looked for a cellular model that mimics the in vivo behavior of melanoblasts, and that allows functional studies of their migration. We report here that wild-type embryonic stem (ES) cells are able to follow the ventral and dorsolateral neural crest pathways after being grafted into chicken embryos. By contrast, a mutant ES cell line deficient for beta1 integrin subunits, proteins involved in cell-extracellular interactions, had a severely impaired migratory behavior. Interestingly, ES cells deficient for Kit, the tyrosine kinase receptor for the stem cell factor (SCF), behaved similarly to wild-type ES cells. Thus, grafting mouse ES cells into chicken embryos provides a new cellular system that allows both in vitro and in vivo studies of the molecular mechanisms controlling dorsolateral migration.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oxidorreductasas
/
Glicoproteínas de Membrana
/
Movimiento Celular
/
Proteínas Proto-Oncogénicas c-kit
/
Melanocitos
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Mech Dev
Asunto de la revista:
EMBRIOLOGIA
Año:
1999
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Irlanda