Direct connection of supercritical fluid extraction and supercritical fluid chromatography as a rapid quantitative method for capsaicinoids in placentas of Capsicum.
J Agric Food Chem
; 47(11): 4665-8, 1999 Nov.
Article
en En
| MEDLINE
| ID: mdl-10552868
The fruits of Capsicum annuum L. are used worldwide as chili peppers and in folk medicines. The pungent components of C. annuum, which are irritants, are called capsaicinoids (CAPS), and the most abundant components are capsaicin, dihydrocapsaicin, and nordihydrocapsaicin. To analyze CAPS in the placentas of Capsicum fruits rapidly and safely, we used a directly connected system of supercritical fluid extraction and supercritical fluid chromatography (SFE/SFC). As a column for SFE/SFC, only a silica-type column was found to be suitable. The CAPS contents in placentas of C. annuum cv. Jalapeno (hot type) and C. annuum cv. Shishitoh (less-hot type) determined by the SFE/SFC method agreed well with those in the range of 0-13.81 mg g(-1) fr. wt determined by the usual extraction-HPLC method. The SFE/SFC method has the advantages of no need for pretreatment and no (or minimal) need for organic solvents. We conclude that this method is useful as a rapid (20 min) and safe screening test for the pungency of various Capsicum fruits.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plantas Medicinales
/
Capsicum
/
Capsaicina
/
Cromatografía Liquida
Idioma:
En
Revista:
J Agric Food Chem
Año:
1999
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Estados Unidos