Induction of E6/E7 expression in cottontail rabbit papillomavirus latency following UV activation.
Virology
; 263(2): 388-94, 1999 Oct 25.
Article
en En
| MEDLINE
| ID: mdl-10544111
Latent human papillomavirus (HPV) infections are widespread in the genital and respiratory tracts and are a source of recurrent disease. This study used a cottontail rabbit papillomavirus (CRPV) model to determine the presence of E1, E6, and E7 transcripts in latent infection and to determine the temporal change in transcripts following UV activation. We found E1 transcripts in all latently infected sites but no detectable E6 and E7 transcripts, consistent with our earlier studies of HPV6/11 latency. These results suggest that this transcription pattern is broadly characteristic of latent papillomavirus infections. E6/E7 transcripts were detectable within 1 week of irradiation, with maximal induction (approximately 40% of sites) at 2 weeks postirradiation. Papillomas were induced in approximately 26% of irradiated sites after a 3- to 5-week lag. Sites that did not form papillomas by 3 months after irradiation were CRPV DNA positive but E6/E7 RNA negative. Thus, only a subset of latent infections can be induced to express E6/E7 transcripts and form papillomas. We propose that CRPV can be used to study the molecular processes regulating papillomavirus activation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Activación Viral
/
Regulación Viral de la Expresión Génica
/
Proteínas Oncogénicas Virales
/
Latencia del Virus
/
Papillomavirus del Conejo de Rabo Blanco
/
Infecciones por Papillomavirus
/
Genes Virales
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Virology
Año:
1999
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos