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Age-related changes in alpha-tocopherol dynamics with relation to lipid hydroperoxide content and fluidity of rat erythrocyte membrane.
Yanagawa, K; Takeda, H; Egashira, T; Sakai, K; Takasaki, M; Matsumiya, T.
Afiliación
  • Yanagawa K; Department of Pharmacology, Tokyo Medical University, Japan.
J Gerontol A Biol Sci Med Sci ; 54(9): B379-83, 1999 Sep.
Article en En | MEDLINE | ID: mdl-10536642
Age-related changes in alpha-tocopherol dynamics in plasma and erythrocyte membranes of 10- to 120-week-old rats were investigated by high-performance liquid chromatography (HPLC) with redox detection mode. Furthermore, changes in lipid hydroperoxide content and fluidity of erythrocyte membrane with age were assessed using chemiluminescence-HPLC and Fourier transform infrared spectrophotometer, respectively. A slight increase in the alpha-tocopherolquinone/alpha-tocopherol ratio in erythrocyte membrane and a decrease in the alpha-tocopherol in erythrocyte membrane/alpha-tocopherol in plasma ratio were observed. A significant increase in lipid hydroperoxide content and a marked decrease in the fluidity of erythrocyte membrane were seen with age. These findings suggest that alpha-tocopherol uptake in erythrocyte membrane declines, and utilization rate of alpha-tocopherol in erythrocyte membrane increases age-dependently. These changes, which enhanced lipid peroxidation and consequently reduced membrane fluidity, may be caused by the impairment of this transfer mechanism.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vitamina E / Envejecimiento / Membrana Eritrocítica / Peróxidos Lipídicos / Fluidez de la Membrana Límite: Animals Idioma: En Revista: J Gerontol A Biol Sci Med Sci Asunto de la revista: GERIATRIA Año: 1999 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vitamina E / Envejecimiento / Membrana Eritrocítica / Peróxidos Lipídicos / Fluidez de la Membrana Límite: Animals Idioma: En Revista: J Gerontol A Biol Sci Med Sci Asunto de la revista: GERIATRIA Año: 1999 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos