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PCR-based quantitation of Cryptosporidium parvum in municipal water samples.
Chung, E; Aldom, J E; Carreno, R A; Chagla, A H; Kostrzynska, M; Lee, H; Palmateer, G; Trevors, J T; Unger, S; Xu, R; De Grandis, S A.
Afiliación
  • Chung E; Department of Environmental Biology, University of Guelph, Ontario, Canada.
J Microbiol Methods ; 38(1-2): 119-30, 1999 Oct.
Article en En | MEDLINE | ID: mdl-10520592
A PCR method for the quantitation of Cryptosporidium parvum oocysts in municipal drinking water samples was investigated. Quantitative PCR uses an internal standard (IS) template with unknown target numbers to compare to standards of known concentrations in a standard curve. The IS template was amplified using the same primers used to amplify a portion of a 358 bp gene fragment that encodes a repetitive oocyst wall protein in C. parvum. Municipal water samples spiked with known numbers of C. parvum oocysts were tested by quantitative PCR using the IS and the Digene SHARP Signal System Assay for PCR product detection. The absorbance readings for target DNA and IS templates versus the number of molecules of the target DNA were plotted to generate standard curves for estimating oocyst numbers. The method allowed the quantitation of oocysts from log 3 to log 5 spiked into municipal water samples.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microbiología del Agua / Cryptosporidium parvum Límite: Animals Idioma: En Revista: J Microbiol Methods Año: 1999 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Países Bajos
Buscar en Google
Añadir a Mi BVS
Imprimir
XML
PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microbiología del Agua / Cryptosporidium parvum Límite: Animals Idioma: En Revista: J Microbiol Methods Año: 1999 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Países Bajos