Naturally induced secretions of the potato cyst nematode co-stimulate the proliferation of both tobacco leaf protoplasts and human peripheral blood mononuclear cells.

Goverse, A; Rouppe van der Voort, J; Roppe van der Voort, C; Kavelaars, A; Smant, G; Schots, A; Bakker, J; Helder, J

Goverse, A; Rouppe van der Voort, J; Roppe van der Voort, C; Kavelaars, A; Smant, G; Schots, A; Bakker, J; Helder, J.

Afiliación

Goverse A; Graduate School for Experimental Plant Sciences, Laboratory for Nematology, Wageningen University, The Netherlands. aska.goverse@medew.nema.wau.nl

Mol Plant Microbe Interact ; 12(10): 872-81, 1999 Oct.

Article en En | MEDLINE | ID: mdl-10517027

RESUMEN

Naturally induced secretions from infective juveniles of the potato cyst nematode Globodera rostochiensis co-stimulate the proliferation of tobacco leaf protoplasts in the presence of the synthetic phytohormones alpha-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP). With the use of a protoplast-based bioassay, a low-molecular-weight peptide(s) (< 3 kDa) was shown to be responsible for the observed effect. This mitogenic oligopeptide(s) is functionally dissimilar to auxin and cytokinin and, in addition, it does not change the sensitivity of the protoplasts toward these phytohormones. In combination with the mitogen phytohemagglutinin (PHA), cyst nematode secretions also co-stimulated mitogenesis in human peripheral blood mononuclear cells (PBMC). The stimulation of plant cells isolated from nontarget tissue--these nematodes normally invade the roots of potato plants--suggests the activation of a general signal transduction mechanism(s) by an oligopeptide(s) secreted by the nematode. Whether a similar oligopeptide-induced mechanism underlies human PBMC activation remains to be investigated. Reactivation of the cell cycle is a crucial event in feeding cell formation by cyst nematodes. The secretion of a mitogenic low-molecular-weight peptide(s) by infective juveniles of the potato cyst nematode could contribute to the redifferentiation of plant cells into such a feeding cell.

Asunto(s)

Adenina/análogos & derivados; Leucocitos Mononucleares/citología; Ácidos Naftalenoacéticos/farmacología; Nematodos/fisiología; Nicotiana/citología; Reguladores del Crecimiento de las Plantas/farmacología; Plantas Tóxicas; Solanum tuberosum/parasitología; Adenina/farmacología; Animales; Compuestos de Bencilo; División Celular; Humanos; Cinetina; Leucocitos Mononucleares/efectos de los fármacos; Hojas de la Planta; Protoplastos/efectos de los fármacos; Protoplastos/fisiología; Purinas; Nicotiana/efectos de los fármacos; Nicotiana/fisiología

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reguladores del Crecimiento de las Plantas / Plantas Tóxicas / Nicotiana / Solanum tuberosum / Leucocitos Mononucleares / Adenina / Ácidos Naftalenoacéticos / Nematodos Límite: Animals / Humans Idioma: En Revista: Mol Plant Microbe Interact Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA / MICROBIOLOGIA Año: 1999 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos

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