A new assay method of gamma-glutamyltransferase with 4-aminobenzoate hydroxylase from Agaricus bisporus as a coupling enzyme.
Clin Chim Acta
; 287(1-2): 83-97, 1999 Sep.
Article
en En
| MEDLINE
| ID: mdl-10509898
We have developed a new ultraviolet spectrophotometric method for measurement of serum gamma-glutamyltransferase activity. The principle of the method is as follows. Using L-gamma-glutamyl-3-chloro-4-aminobenzoate as the donor substrate, 3-chloro-4-aminobenzoate formed upon transfer of the gamma-glutamyl moiety from the donor substrate to the acceptor substrate glycylglycine is stoichiometrically converted to 3-chloro-4-hydroxyaniline by 4-aminobenzoate hydroxylase from Agaricus bisporus, coupled with the oxidation of NADH to NAD+, and the resulting decrease in absorbance at 340 nm is monitored. The results obtained indicate that there is a good possibility to establish an ultraviolet spectrophotometric method for measurement of serum gamma-glutamyltransferase activity using L-gamma-glutamyl-3-chloro-4-aminobenzoate as the donor substrate and 4-aminobenzoate hydroxylase from Agaricus bisporus as a coupling enzyme.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Agaricus
/
Cromatografía de Afinidad
/
Cromatografía en Gel
/
Gamma-Glutamiltransferasa
/
Oxigenasas de Función Mixta
Límite:
Humans
Idioma:
En
Revista:
Clin Chim Acta
Año:
1999
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Países Bajos