Confocal laser scanning microscopy of calcium dynamics in living cells.

Stricker, S A; Whitaker, M

Stricker, S A; Whitaker, M.

Afiliación

Stricker SA; Department of Biology, University of New Mexico, Albuquerque, New Mexico 87131, USA. sstr@unm.edu

Microsc Res Tech ; 46(6): 356-69, 1999 Sep 15.

Article en En | MEDLINE | ID: mdl-10504213

RESUMEN

Confocal laser scanning microscopy (CLSM) is widely used to monitor intracellular calcium levels in living cells loaded with calcium-sensitive fluorophores. This review examines the basic advantages and limitations of CLSM in in vivo imaging analyses of calcium dynamics. The benefits of utilizing ratioed images and dextran-conjugated fluorophores are addressed, and practical aspects of handling confocal datasets are outlined. After considering some relatively new microscopical methods that can be used in conjunction with conventional CLSM, possible future applications of confocal techniques in analyses of intracellular calcium dynamics are discussed.

Asunto(s)

Calcio/análisis; Microscopía Confocal; Animales; Calibración; Colorantes; Dextranos; Colorantes Fluorescentes; Humanos

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calcio / Microscopía Confocal Límite: Animals / Humans Idioma: En Revista: Microsc Res Tech Asunto de la revista: DIAGNOSTICO POR IMAGEM Año: 1999 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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