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Acute toxicity of an anti-Fas antibody in mice.
Kakinuma, C; Takagaki, K; Yatomi, T; Nakamura, N; Nagata, S; Uemura, A; Shibutani, Y.
Afiliación
  • Kakinuma C; Toxicology Laboratory, Mochida Pharmaceutical Co., Ltd., Fujieda, Shizuoka, Japan.
Toxicol Pathol ; 27(4): 412-20, 1999.
Article en En | MEDLINE | ID: mdl-10485821
By histopathologic examination of various organs in 3 normal strains, C3H/HeN, ICR, and DBA/1J, of mice treated intravenously once with anti-Fas antibody (Jo2), we failed to determine any target organ, except the liver, responsible for the acute lethality induced by the Fas/anti-Fas antibody interaction. However, we could show the presence of Fas-mediated apoptosis in other organs aside from the liver and normal mouse strain differences in susceptibility to anti-Fas antibody. Among these strains, C3H/HeN was the most susceptible to the antibody, followed by ICR and DBA/1J. We observed Fas-mediated apoptosis in the liver, spleen, thymus, lymph nodes, Peyer's patch, intestine, skin, coagulation glands, ovary, uterus, and vagina in all 3 strains and additionally in the epididymides and seminal vesicles in the DBA/1J strain. We also demonstrated that Fas-mediated apoptosis of small lymphocytes in the mantle zone of splenic lymphatic follicles preceded that of the hepatocytes or thymic cells. Since cellular damage was most severe in the liver among all the apoptotic organs in the 3 mouse strains, liver injury induced by anti-Fas antibody is speculated to play a significant role in the death.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptor fas / Anticuerpos Monoclonales Límite: Animals Idioma: En Revista: Toxicol Pathol Año: 1999 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptor fas / Anticuerpos Monoclonales Límite: Animals Idioma: En Revista: Toxicol Pathol Año: 1999 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos