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Identification of amplified genes in a patient with acute myeloid leukemia and double minute chromosomes.
Crossen, P E; Morrison, M J; Rodley, P; Cochrane, J; Morris, C M.
Afiliación
  • Crossen PE; Cytogenetic and Molecular Oncology Unit, Christchurch Hospital, New Zealand.
Cancer Genet Cytogenet ; 113(2): 126-33, 1999 Sep.
Article en En | MEDLINE | ID: mdl-10484978
A case of acute myeloid leukemia (M2) with double minute chromosomes and complex karyotypic abnormalities was analyzed cytogenetically and molecularly. Comparative genomic hybridization (CGH) showed that the 8q24 region that contains the MYC oncogene was not amplified. Instead, amplification of chromosomal regions 11q23-->qter and 9p11-->pter was identified. Southern blot analysis confirmed the CGH findings and showed that the ETS1, FLI1, SRPR, NFRKB, and KCNJ5 genes located at 11q23-->24 were amplified, whereas the MLL at 11q23 was not amplified. Additionally, the IFN beta 1 and CDKN2A genes at 9p were amplified, but to a lesser degree. This is the first example of a case of acute myeloid leukemia with double minute chromosomes that has not involved amplification of either the MYC or the MLL genes.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucemia Mieloide / Amplificación de Genes / Aberraciones Cromosómicas / Canales de Potasio de Rectificación Interna Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Aged / Humans / Male Idioma: En Revista: Cancer Genet Cytogenet Año: 1999 Tipo del documento: Article País de afiliación: Nueva Zelanda Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucemia Mieloide / Amplificación de Genes / Aberraciones Cromosómicas / Canales de Potasio de Rectificación Interna Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Aged / Humans / Male Idioma: En Revista: Cancer Genet Cytogenet Año: 1999 Tipo del documento: Article País de afiliación: Nueva Zelanda Pais de publicación: Estados Unidos