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Modulation of protein kinase C isoforms by PAF in cerebral cortex.
Calcerrada, M C; Pérez-Alvarez, M J; Catalán, R E; Martínez, A M.
Afiliación
  • Calcerrada MC; Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense de Madrid, Spain.
Prostaglandins Other Lipid Mediat ; 58(1): 19-27, 1999 Aug.
Article en En | MEDLINE | ID: mdl-10482284
The effect of platelet activating factor (PAF) on subcellular distribution of protein kinase C isoforms in rat cerebral cortex was investigated. PAF induced an increase in levels of protein kinase C epsilon and gamma in membrane fraction. Results also indicate that PAF induced an increase in protein kinase C delta levels in both cytosolic and membrane fraction. This effect is possibly due to an increase in enzyme synthesis, as indicated by the results obtained from the experiments performed in the presence of cycloheximide and actinomycin. All the effects induced by PAF were time- and dose-dependent, and were mediated through the activation of PAF receptor. These findings indicate that the three isoforms may be involved in signal transduction of PAF in the brain.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteína Quinasa C / Factor de Activación Plaquetaria / Corteza Cerebral / Isoenzimas Límite: Animals Idioma: En Revista: Prostaglandins Other Lipid Mediat Asunto de la revista: ENDOCRINOLOGIA Año: 1999 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteína Quinasa C / Factor de Activación Plaquetaria / Corteza Cerebral / Isoenzimas Límite: Animals Idioma: En Revista: Prostaglandins Other Lipid Mediat Asunto de la revista: ENDOCRINOLOGIA Año: 1999 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos