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Inhibition of protein tyrosine phosphatases blocks calcium-induced activation of metaphase II-arrested oocytes of Xenopus laevis.
Bodart, J F; Béchard, D; Bertout, M; Rousseau, A; Gannon, J; Vilain, J P; Flament, S.
Afiliación
  • Bodart JF; Centre de Biologie Cellulaire, Laboratoire de Biologie du Développement, UPRES EA 1033, Université de Lille 1, SN3, F-59655, Villeneuve d'Ascq, France.
FEBS Lett ; 457(2): 175-8, 1999 Aug 27.
Article en En | MEDLINE | ID: mdl-10471773
We have studied the effect of a protein tyrosine phosphatases (PTP) inhibitor on calcium-induced activation of Xenopus laevis oocytes arrested at metaphase II. Ammonium molybdate microinjection blocked pronucleus formation following A23187 treatment while cortical granules still underwent exocytosis. Pronuclei still occurred in ammonium molybdate-injected oocytes following 6-DMAP addition. Changes that usually occurred following A23187 exposure were inhibited in the presence of ammonium molybdate in the oocyte: MAPK dephosphorylation, p34(cdc2) rephosphorylation and cyclin B2 and p39(mos) proteolysis. These results suggest that a PTP is involved in the activation of the ubiquitin-dependent degradation machinery.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calcio / Proteínas Tirosina Fosfatasas / Metafase Límite: Animals Idioma: En Revista: FEBS Lett Año: 1999 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido
Buscar en Google
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PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calcio / Proteínas Tirosina Fosfatasas / Metafase Límite: Animals Idioma: En Revista: FEBS Lett Año: 1999 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido