Expression in cattle of epitopes of a heterologous virus using a recombinant rinderpest virus.
J Gen Virol
; 80 ( Pt 8): 2031-2039, 1999 Aug.
Article
en En
| MEDLINE
| ID: mdl-10466801
We have investigated the bovine immune response to heterologous proteins expressed using a recombinant rinderpest virus (RPV). A new gene unit was created in a cDNA copy of the genome of the vaccine strain of RPV, and an open reading frame inserted that encodes the polymerase (3Dpol) and parts of the capsid protein VP1 from foot-and-mouth disease virus (FMDV). Infectious recombinant RPV was rescued and shown to express the FMDV-derived protein at good levels in infected cells. The rescued virus was only slightly more attenuated in tissue culture than the original virus. Cattle infected with this recombinant generated a normal immune response to RPV, and were protected from lethal challenge by that virus. Experimental animals showed a specific delayed-type hypersensitivity response to FMDV 3Dpol, similar to that seen in FMDV infection; however, no antibodies were detected recognizing either of the components of the FMDV-derived protein, nor was any proliferative response to these epitopes found in isolated peripheral blood lymphocytes from infected animals. No protection was seen against FMDV infection.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virus de la Peste Bovina
/
ARN Polimerasas Dirigidas por ADN
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Cápside
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Proteínas no Estructurales Virales
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Aphthovirus
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Epítopos de Linfocito B
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Vectores Genéticos
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Antígenos Virales
Límite:
Animals
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Humans
Idioma:
En
Revista:
J Gen Virol
Año:
1999
Tipo del documento:
Article
Pais de publicación:
Reino Unido