Isolation of indole alkaloids from Catharanthus roseus by centrifugal partition chromatography in the pH-zone refining mode.

Renault, J H; Nuzillard, J M; Le Crouérour, G; Thépenier, P; Zèches-Hanrot, M; Le Men-Olivier, L

Renault, J H; Nuzillard, J M; Le Crouérour, G; Thépenier, P; Zèches-Hanrot, M; Le Men-Olivier, L.

Afiliación

Renault JH; Laboratoire de Pharmacognosie, UPRES-A CNRS 6013, CPCBAI, Reims, France.

J Chromatogr A ; 849(2): 421-31, 1999 Jul 23.

Article en En | MEDLINE | ID: mdl-10457440

RESUMEN

Centrifugal partition chromatography (CPC) in the pH-zone refining mode allowed a preparative and efficient isolation of vindoline, vindolinine, catharanthine and vincaleukoblastine from a crude mixture of Catharanthus roseus alkaloids. The separation protocol was tested with a synthetic mixture of vindoline, catharanthine and vincaleukoblastine. The fraction content was analyzed and the results compared with theoretical chromatograms obtained by numerical simulation. The increase in injected sample mass results in an improvement of the purity of the isolated compounds. This observation, confirmed by theory, is of prime importance for the development of preparative pH-zone refining CPC as a preparative separation method.

Asunto(s)

Cromatografía/métodos; Indoles/aislamiento & purificación; Alcaloides de la Vinca/aislamiento & purificación; Concentración de Iones de Hidrógeno

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alcaloides de la Vinca / Cromatografía / Indoles Idioma: En Revista: J Chromatogr A Año: 1999 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Países Bajos

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