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Granulocyte and plasma cytokine activity in acute cadmium intoxication in rats.
Kataranovski, M; Kataranovski, D; Savic, D; Jovcic, G; Bogdanovic, Z; Jovanovic, T.
Afiliación
  • Kataranovski M; Institute for Medical Research, Military Medical Academy, Belgrade, Yugoslavia.
Physiol Res ; 47(6): 453-61, 1998.
Article en En | MEDLINE | ID: mdl-10453753
Changes in the number and ex vivo function of peripheral blood neutrophils were investigated following intraperitoneal administration of cadmium-chloride in rats. Besides a dose-dependent increase in the number of peripheral blood neutrophils, changes were found in the functional state of isolated polymorphonuclear leukocytes (PMNs). Increased spontaneous adhesion and activation, and TNF activity in a conditioned medium were observed in cultures of granulocytes in comparison to granulocytes from control (saline-treated) animals. Increased levels of plasma activity of inflammatory cytokines, tumor necrosis factor (TNF) and interleukin-6 (IL-6) were noted following cadmium administration. Cytological signs of pulmonary inflammation were revealed histologically and the majority of neutrophils recovered from the lungs by enzyme digestion exhibited a capacity of nitroblue tetrazolium (NBT) reduction. Our data demonstrate that acute cadmium intoxication leads to a systemic inflammatory response characterized by numerical and functional changes in the granulocyte compartment and to increased levels of inflammation-related cytokine activity in the circulation. Correlations between the increased number of peripheral blood neutrophils and IL-6 plasma activity (r=0.776, p<0.00001) and the number of neutrophils recovered from the lung tissue (r=0.893, p<0.00001) suggested that systemic cadmium-induced inflammation might be involved in the pulmonary toxicity of cadmium.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Intoxicación por Cadmio / Interleucina-6 / Factor de Necrosis Tumoral alfa / Neutrófilos Límite: Animals Idioma: En Revista: Physiol Res Asunto de la revista: FISIOLOGIA Año: 1998 Tipo del documento: Article País de afiliación: Yugoslavia Pais de publicación: República Checa
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Intoxicación por Cadmio / Interleucina-6 / Factor de Necrosis Tumoral alfa / Neutrófilos Límite: Animals Idioma: En Revista: Physiol Res Asunto de la revista: FISIOLOGIA Año: 1998 Tipo del documento: Article País de afiliación: Yugoslavia Pais de publicación: República Checa