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Visualisation of AMPA binding sites in the brain stem of normotensive and hypertensive rats.
Ashworth-Preece, M A; Chen, F; Jarrott, B; Lawrence, A J.
Afiliación
  • Ashworth-Preece MA; Department of Pharmacology, Monash University, Wellington Road, Clayton, Victoria 3168, Australia.
Brain Res ; 834(1-2): 186-9, 1999 Jul 10.
Article en En | MEDLINE | ID: mdl-10407113
The present study has employed in vitro receptor autoradiography with (S)-[(3)H]-5-fluorowillardiine (10 nM) to visualise the presence of alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) binding sites in the brain stems of adult (16-18 weeks) normotensive (Wistar-Kyoto (WKY) and Don Ryu (DRY)) and Spontaneously Hypertensive (SHR) rats. Similar topographic distribution and density of (S)-[(3)H]-5-fluorowillardiine binding was observed in the nucleus tractus solitarius (NTS) of all three strains. Specific (S)-[(3)H]-5-fluorowillardiine binding sites were also visualised in sections of nodose ganglion from adult WKY rats, demonstrating that vagal afferent perikarya possess AMPA binding sites. However, while unilateral vagal deafferentation did not result in a significant decrease in binding site density in the caudal half of the rat NTS, the visualisation of AMPA binding sites on the nodose ganglion is consistent with the existence of a population of binding sites on vagal afferent terminals. In the caudal half of the rat NTS, AMPA binding sites appear to be predominantly postsynaptic in nature.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tronco Encefálico / Ácido alfa-Amino-3-hidroxi-5-metil-4-isoxazol Propiónico / Hipertensión Límite: Animals Idioma: En Revista: Brain Res Año: 1999 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Países Bajos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tronco Encefálico / Ácido alfa-Amino-3-hidroxi-5-metil-4-isoxazol Propiónico / Hipertensión Límite: Animals Idioma: En Revista: Brain Res Año: 1999 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Países Bajos