Identification of human rotavirus strains with the P[14] genotype by PCR.

Arista, S; Vizzi, E; Alaimo, C; Palermo, D; Cascio, A

Arista, S; Vizzi, E; Alaimo, C; Palermo, D; Cascio, A.

Afiliación

Arista S; Dipartimento di Igiene e Microbiologia, Università di Palermo," Palermo, Italy.

J Clin Microbiol ; 37(8): 2706-8, 1999 Aug.

Article en En | MEDLINE | ID: mdl-10405427

RESUMEN

A seminested PCR typing assay has been extended to identify rotavirus strains with the P[14] genotype. The specificity of the method was confirmed by Southern hybridization and by restriction analysis with the enzyme AluI. One out of four human rotavirus (HRV) strains with unusual subgroup-electropherotype linkage but none out of 50 HRV strains with usual linkage was typed as P[14].

Asunto(s)

Genoma Viral; Rotavirus/genética; Cápside/genética; Humanos; Reacción en Cadena de la Polimerasa; Rotavirus/clasificación; Rotavirus/aislamiento & purificación

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Genoma Viral / Rotavirus Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: J Clin Microbiol Año: 1999 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos

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