Biochemical studies on leukocyte and fibroblast human beta-galactosidase.
Clin Biochem
; 32(3): 167-70, 1999 Apr.
Article
en En
| MEDLINE
| ID: mdl-10383075
OBJECTIVES: Some biochemical characteristics of the human leukocyte and fibroblast beta-galactosidase were studied. DESIGN AND METHODS: Leukocyte and fibroblast enzyme activity was determined fluorometricaly using 4-methylumbelliferyl-beta-D-galactoside as artificial substrate. Optimum pH, Km, Vmax and thermostability of the enzyme at 42 degrees C were determined. RESULTS: The leukocyte and fibroblast enzyme has an optimum pH at 4.2, which is in agreement with the lysosomal origin of the enzyme. The Km of the enzyme was 0.62 in leukocytes and 0.67 in fibroblasts, and Vmax was 289.9 nmol/h/mg of protein and 1779.2 nmol/h/mg of protein in the two tissues, respectively. When fibroblast or leukocyte beta-galactosidase was pre-incubated at 42 degrees C, it did not retain its activity because the residual activity after 80 minutes of pre-incubation at this temperature was lower than 30% of the initial activity both in leukocytes and fibroblasts. CONCLUSIONS: This was the first study of Km, Vmax and thermostability of beta-galactosidase performed on leukocytes and provided data for a better characterization of the enzyme beta-galactosidase, allowing the improvement of the analytical conditions.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Beta-Galactosidasa
/
Leucocitos
Límite:
Humans
Idioma:
En
Revista:
Clin Biochem
Año:
1999
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Estados Unidos