A Saccharomyces cerevisiae G-protein coupled receptor, Gpr1, is specifically required for glucose activation of the cAMP pathway during the transition to growth on glucose.

Kraakman, L; Lemaire, K; Ma, P; Teunissen, A W; Donaton, M C; Van Dijck, P; Winderickx, J; de Winde, J H; Thevelein, J M

Kraakman, L; Lemaire, K; Ma, P; Teunissen, A W; Donaton, M C; Van Dijck, P; Winderickx, J; de Winde, J H; Thevelein, J M.

Afiliación

Kraakman L; Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit Leuven, Institute of Botany and Microbiology.

Mol Microbiol ; 32(5): 1002-12, 1999 Jun.

Article en En | MEDLINE | ID: mdl-10361302

RESUMEN

In the yeast Saccharomyces cerevisiae the accumulation of cAMP is controlled by an elaborate pathway. Only two triggers of the Ras adenylate cyclase pathway are known. Intracellular acidification induces a Ras-mediated long-lasting cAMP increase. Addition of glucose to cells grown on a non-fermentable carbon source or to stationary-phase cells triggers a transient burst in the intracellular cAMP level. This glucose-induced cAMP signal is dependent on the G alpha-protein Gpa2. We show that the G-protein coupled receptor (GPCR) Gpr1 interacts with Gpa2 and is required for stimulation of cAMP synthesis by glucose. Gpr1 displays sequence homology to GPCRs of higher organisms. The absence of Gpr1 is rescued by the constitutively activated Gpa2Val-132 allele. In addition, we isolated a mutant allele of GPR1, named fil2, in a screen for mutants deficient in glucose-induced loss of heat resistance, which is consistent with its lack of glucose-induced cAMP activation. Apparently, Gpr1 together with Gpa2 constitute a glucose-sensing system for activation of the cAMP pathway. Deletion of Gpr1 and/or Gpa2 affected cAPK-controlled features (levels of trehalose, glycogen, heat resistance, expression of STRE-controlled genes and ribosomal protein genes) specifically during the transition to growth on glucose. Hence, an alternative glucose-sensing system must signal glucose availability for the Sch9-dependent pathway during growth on glucose. This appears to be the first example of a GPCR system activated by a nutrient in eukaryotic cells. Hence, a subfamily of GPCRs might be involved in nutrient sensing.

Asunto(s)

AMP Cíclico/metabolismo; Subunidades alfa de la Proteína de Unión al GTP; Glucosa/metabolismo; Proteínas de Unión al GTP Heterotriméricas; Receptores de Superficie Celular/metabolismo; Receptores Acoplados a Proteínas G; Proteínas de Saccharomyces cerevisiae; Saccharomyces cerevisiae/metabolismo; Secuencia de Aminoácidos; Northern Blotting; Proteínas Quinasas Dependientes de AMP Cíclico/metabolismo; Proteínas Fúngicas/metabolismo; Proteínas de Unión al GTP/metabolismo; Regulación Fúngica de la Expresión Génica; Genes Fúngicos; Datos de Secuencia Molecular; Mutación; Receptores de Superficie Celular/genética; Saccharomyces cerevisiae/genética; Saccharomyces cerevisiae/crecimiento & desarrollo; Transducción de Señal

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / AMP Cíclico / Receptores de Superficie Celular / Proteínas de Unión al GTP Heterotriméricas / Proteínas de Saccharomyces cerevisiae / Subunidades alfa de la Proteína de Unión al GTP / Receptores Acoplados a Proteínas G / Glucosa Idioma: En Revista: Mol Microbiol Asunto de la revista: BIOLOGIA MOLECULAR / MICROBIOLOGIA Año: 1999 Tipo del documento: Article Pais de publicación: Reino Unido

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links