Ornithine alpha-ketoglutarate counteracts the decrease of liver cytochrome P-450 content in burned rats.
Nutrition
; 15(5): 379-83, 1999 May.
Article
en En
| MEDLINE
| ID: mdl-10355851
The effect of ornithine alpha-ketoglutarate (OKG) on cytochrome P-450 enzyme activities was studied in a well-defined model of injury (burn followed by fasting then subsequent hypocaloric diet) administered to young rats for 3 d. Hepatic microsomes were prepared by ultracentrifugation and levels of cytochromes P-450 were determined spectrophotometrically. The activities of ethoxy-resorufin-O-deethylase (EROD), benzyloxy-resorufin-O-dealkylase (BROD), and erythromycin demethylase were measured as markers of P-450 1A, 2A, and 3A isotypes respectively. The level of total hepatic microsomal proteins (8 mg/mL) remained constant. The level of cytochrome P-450 (1.14+/-0.08 nmol/mg microsomal proteins) was decreased by a hypocaloric diet (23%, P = 0.003) and burn further enhanced this phenomenon (15%, P = 0.03). Both healthy and burned rats receiving OKG showed the same level of cytochrome P-450 as the rats fed ad libitum. OKG supplementation counteracted the enhancement (40%) of EROD activity induced by hypocaloric diet but did not influence BROD and erythromycin demethylase activities. OKG sustained cytochrome P-450 levels in rats fed a hypocaloric diet, even after burning. These findings indicate that OKG may favor drug metabolism in this injured population.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ornitina
/
Quemaduras
/
Microsomas Hepáticos
/
Hidrocarburo de Aril Hidroxilasas
/
Sistema Enzimático del Citocromo P-450
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Nutrition
Asunto de la revista:
CIENCIAS DA NUTRICAO
Año:
1999
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Estados Unidos