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Regulation of Ca-sensitive inactivation of a 1-type Ca2+ channel by specific domains of beta subunits.
Cens, T; Restituito, S; Charnet, P.
Afiliación
  • Cens T; CRBM, CNRS UPR 1086, IFR 24, Montpellier, France.
FEBS Lett ; 450(1-2): 17-22, 1999 Apr 30.
Article en En | MEDLINE | ID: mdl-10350049
Ca2+ channel auxiliary beta subunits have been shown to modulate voltage-dependent inactivation of various types of Ca2+ channels. The beta1 and beta2 subunits, that are differentially expressed with the L-type alpha1 Ca2+ channel subunit in heart, muscle and brain, can specifically modulate the Ca2+-dependent inactivation kinetics. Their expression in Xenopus oocytes with the alpha1C subunit leads, in both cases, to biphasic Ca2+ current decays, the second phase being markedly slowed by expression of the beta2 subunit. Using a series of beta subunit deletion mutants and chimeric constructs of beta1 and beta2 subunits, we show that the inhibitory site located on the amino-terminal region of the beta2a subunit is the major element of this regulation. These results thus suggest that different splice variants of the beta2 subunit can modulate, in a specific way, the Ca2+ entry through L-type Ca2+ channels in different brain or heart regions.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Canales de Calcio / Calcio Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: FEBS Lett Año: 1999 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Canales de Calcio / Calcio Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: FEBS Lett Año: 1999 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido