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The effect of erdosteine and its active metabolite on reactive oxygen species production by inflammatory cells.
Miyake, K; Kaise, T; Hosoe, H; Akuta, K; Manabe, H; Ohmori, K.
Afiliación
  • Miyake K; Drug Development Research Laboratories, Pharmaceutical Research Institute, Kyowa Hakko Kogyo Co., Ltd., Sizuoka, Japan.
Inflamm Res ; 48(4): 205-9, 1999 Apr.
Article en En | MEDLINE | ID: mdl-10344471
OBJECTIVE: We examined the effect of erdosteine (KW-9144), an expectorant, and related compounds on inflammatory cell-derived reactive oxygen species which are involved in airway inflammation. METHODS: Neutrophils were isolated from peritoneal lavages of casein-injected rats and from peripheral blood of healthy human donors. Eosinophils were isolated from peritoneal lavages of horse serum-injected guinea pigs. These cells were stimulated with phorbol 12-myristate 13-acetate (PMA) and the production of reactive oxygen species was measured with luminol-dependent chemiluminescence (LDCL). RESULTS: M1, an active metabolite of erdosteine, significantly inhibited PMA-induced LDCL of the all cell populations with treatment before stimulation. The effects of S-carboxymethylcysteine (S-CMC), ambroxol and N-acetylcysteine (NAC) on the LDCL response were weaker than those of M1. Furthermore, PMA-induced LDCL was decreased by posttreatment with M1. CONCLUSION: These results suggest that M (an active metabolite of erdosteine) may exert an antiinflammatory effect by scavenging inflammatory cells-derived reactive oxygen species.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tioglicolatos / Tiofenos / Especies Reactivas de Oxígeno / Eosinófilos / Neutrófilos Límite: Animals / Humans / Male Idioma: En Revista: Inflamm Res Asunto de la revista: ALERGIA E IMUNOLOGIA / PATOLOGIA Año: 1999 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tioglicolatos / Tiofenos / Especies Reactivas de Oxígeno / Eosinófilos / Neutrófilos Límite: Animals / Humans / Male Idioma: En Revista: Inflamm Res Asunto de la revista: ALERGIA E IMUNOLOGIA / PATOLOGIA Año: 1999 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Suiza