Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp. nov.

Venkateswaran, K; Moser, D P; Dollhopf, M E; Lies, D P; Saffarini, D A; MacGregor, B J; Ringelberg, D B; White, D C; Nishijima, M; Sano, H; Burghardt, J; Stackebrandt, E; Nealson, K H

Venkateswaran, K; Moser, D P; Dollhopf, M E; Lies, D P; Saffarini, D A; MacGregor, B J; Ringelberg, D B; White, D C; Nishijima, M; Sano, H; Burghardt, J; Stackebrandt, E; Nealson, K H.

Afiliación

Venkateswaran K; Center for Great Lakes Studies, University of Wisconsin-Milwaukee 53204, USA. kjvenkat@caltech.edu

Int J Syst Bacteriol ; 49 Pt 2: 705-24, 1999 Apr.

Article en En | MEDLINE | ID: mdl-10319494

RESUMEN

The genus Shewanella has been studied since 1931 with regard to a variety of topics of relevance to both applied and environmental microbiology. Recent years have seen the introduction of a large number of new Shewanella-like isolates, necessitating a coordinated review of the genus. In this work, the phylogenetic relationships among known shewanellae were examined using a battery of morphological, physiological, molecular and chemotaxonomic characterizations. This polyphasic taxonomy takes into account all available phenotypic and genotypic data and integrates them into a consensus classification. Based on information generated from this study and obtained from the literature, a scheme for the identification of Shewanella species has been compiled. Key phenotypic characteristics were sulfur reduction and halophilicity. Fatty acid and quinone profiling were used to impart an additional layer of information. Molecular characterizations employing small-subunit 16S rDNA sequences were at the limits of resolution for the differentiation of species in some cases. As a result, DNA-DNA hybridization and sequence analyses of a more rapidly evolving molecule (gyrB gene) were performed. Species-specific PCR probes were designed for the gyrB gene and used for the rapid screening of closely related strains. With this polyphasic approach, in addition to the ten described Shewanella species, two new species, Shewanella oneidensis and 'Shewanella pealeana', were recognized; Shewanella oneidensis sp. nov. is described here for the first time.

Asunto(s)

Infecciones por Bacterias Gramnegativas/microbiología; Bacilos Gramnegativos Anaerobios Facultativos/clasificación; Filogenia; Animales; Benzoquinonas/análisis; Girasa de ADN; ADN-Topoisomerasas de Tipo II/genética; ADN Bacteriano/química; ADN Bacteriano/genética; ADN Ribosómico/química; ADN Ribosómico/genética; Microbiología Ambiental; Ácidos Grasos/análisis; Genes de ARNr; Genotipo; Bacilos Gramnegativos Anaerobios Facultativos/citología; Bacilos Gramnegativos Anaerobios Facultativos/genética; Bacilos Gramnegativos Anaerobios Facultativos/fisiología; Humanos; Datos de Secuencia Molecular; Fenotipo; ARN Ribosómico 16S/genética; Análisis de Secuencia de ADN; Especificidad de la Especie

Palabras clave

NASA Discipline Exobiology; Non-NASA Center

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Infecciones por Bacterias Gramnegativas / Bacilos Gramnegativos Anaerobios Facultativos Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Int J Syst Bacteriol Año: 1999 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

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