Discrimination between ribonuclease H- and ribonuclease L-mediated RNA degradation by 2'-O-methylated 2-5A-antisense oligonucleotides.

Cramer, H; Player, M R; Torrence, P F

Cramer, H; Player, M R; Torrence, P F.

Afiliación

Cramer H; Section on Biomedical Chemistry, Laboratory of Medicinal Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0805, USA.

Bioorg Med Chem Lett ; 9(7): 1049-54, 1999 Apr 05.

Article en En | MEDLINE | ID: mdl-10230638

RESUMEN

2',5'-Oligoadenylate (2-5A) antisense chimeric oligonucleotides were synthesized containing varying 2'-O-methyl-ribonucleotide substitution patterns in the antisense domain. The ability of these composite oligonucleotides to mediate RNase H- and RNase L-catalyzed RNA degradation showed that these two enzymes have different activation requirements.

Asunto(s)

Nucleótidos de Adenina/genética; Endorribonucleasas/metabolismo; Oligonucleótidos Antisentido/química; Oligorribonucleótidos/genética; ARN/metabolismo; Ribonucleasa H/metabolismo; Secuencia de Bases; Hidrólisis; Metilación

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligorribonucleótidos / ARN / Nucleótidos de Adenina / Oligonucleótidos Antisentido / Ribonucleasa H / Endorribonucleasas Tipo de estudio: Prognostic_studies Idioma: En Revista: Bioorg Med Chem Lett Asunto de la revista: BIOQUIMICA / QUIMICA Año: 1999 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

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