L-leucine auxotrophy in Bifidobacterium globosum.

Mattarelli, P; Biavati, B

Mattarelli, P; Biavati, B.

Afiliación

Mattarelli P; Istituto di Microbiologia Agraria, Bologna, Italy.

New Microbiol ; 22(1): 73-6, 1999 Jan.

Article en En | MEDLINE | ID: mdl-10190121

RESUMEN

The enzymes involved in the biosynthetic pathway of L-leucine were studied in plasmid-negative and plasmid-positive clones derived from the RU 809 strain of the Bifidobacterium globosum species. The growth of plasmid-positive clones in synthetic medium required L-leucine. We have shown that no detectable activity of the beta-isopropylmalate dehydrogenase enzyme was present in plasmid-positive clones, whereas detectable and significant activity of this enzyme was found in plasmid-negative clones. The lack of activity of the beta-isopropylmalate dehydrogenase enzyme is considered responsible for the L-leucine auxotrophy in the plasmid-positive clones.

Asunto(s)

Oxidorreductasas de Alcohol/metabolismo; Bifidobacterium/enzimología; Leucina/metabolismo; 3-Isopropilmalato Deshidrogenasa; Bifidobacterium/crecimiento & desarrollo; Medios de Cultivo/química; Hemiterpenos; Cetoácidos/metabolismo; Plásmidos

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bifidobacterium / Oxidorreductasas de Alcohol / Leucina Idioma: En Revista: New Microbiol Asunto de la revista: MICROBIOLOGIA Año: 1999 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Italia

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