A mathematical model for the generation and control of a pH gradient in an immobilized enzyme system involving acid generation.
Biotechnol Bioeng
; 57(4): 394-408, 1998 Feb 20.
Article
en En
| MEDLINE
| ID: mdl-10099216
An optimal pH control technique has been developed for multistep enzymatic synthesis reactions where the optimal pH differs by several units for each step. This technique separates an acidic environment from a basic environment by the hydrolysis of urea within a thin layer of immobilized urease. With this technique, a two-step enzymatic reaction can take place simultaneously, in proximity to each other, and at their respective optimal pH. Because a reaction system involving an acid generation represents a more challenging test of this pH control technique, a number of factors that affect the generation of such a pH gradient are considered in this study. The mathematical model proposed is based on several simplifying assumptions and represents a first attempt to provide an analysis of this complex problem. The results show that, by choosing appropriate parameters, the pH control technique still can generate the desired pH gradient even if there is an acid-generating reaction in the system.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ácidos
/
Enzimas Inmovilizadas
/
Modelos Químicos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Biotechnol Bioeng
Año:
1998
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos