Induction of prophages of enterohemorrhagic Escherichia coli O157:H7 with norfloxacin.

Matsushiro, A; Sato, K; Miyamoto, H; Yamamura, T; Honda, T

Matsushiro, A; Sato, K; Miyamoto, H; Yamamura, T; Honda, T.

Afiliación

Matsushiro A; The School of Biology-Oriented Science and Technology, Department of Molecular Genetics, Kinki University, Wakayama 649-6493, Osaka 565-0871, Japan.

J Bacteriol ; 181(7): 2257-60, 1999 Apr.

Article en En | MEDLINE | ID: mdl-10094706

RESUMEN

Norfloxacin (NFLX) caused induction of prophages VT1 and VT2 of enterohemorrhagic Escherichia coli O157 at subinhibitory concentrations. In time course experiments, we observed the following sequential events: upon induction, the phage genomes underwent multiplication; the amount of stx genes increased; and subsequently, large quantities of toxins VT1 and VT2 were produced. Further studies showed that the molecular mechanism of prophage induction is closely related to the RecA system since the prophage VT2 was not induced with NFLX in a recA mutant strain.

Asunto(s)

Colifagos/crecimiento & desarrollo; Escherichia coli O157/efectos de los fármacos; Escherichia coli O157/virología; Norfloxacino/farmacología; Activación Viral; Escherichia coli O157/metabolismo; Rec A Recombinasas/metabolismo; Factores de Tiempo

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Activación Viral / Norfloxacino / Colifagos / Escherichia coli O157 Idioma: En Revista: J Bacteriol Año: 1999 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos

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